Submitted to: Pig Veterinary Society International Congress Proceedings
Publication Type: Proceedings
Publication Acceptance Date: 5/1/2004
Publication Date: 7/27/2004
Citation: Solorzano, A., Webby, R.J., Lager, K.M., Janke, B.H., Richt, J. 2004. Identification of swine influenza virus virulence factors using reverse genetics: a novel approach to vaccine development. In: Proceedings of the 18th Congress of the International Pig Veterinary Society, June 27-July 2, 2004, Hamburg, Germany. p. 6.
Technical Abstract: Swine influenza (SI) is an acute respiratory disease of swine caused by type A influenza viruses. Before 1998, mainly "classical" H1N1 SI viruses (SIV) were isolated from swine in the United States (2,3). Since then, antigenetically distinct reassortant H1- and H3-SIV subtypes have been identified as causative agents of respiratory disease in pigs on U.S. farms (5,6,7). The H3N2 SIVs currently circulating in U.S. swine populations are triple reassortant viruses containing avian-like (PA, PB2), swine-like (M, NP, NS) and human-like (HA, NA, PB1) gene segments (6,7). Pigs are suggested to be the "mixing vessel" where reassortment between influenza viruses from different animal species occurs. This may lead to the generation of novel influenza viruses capable of crossing the species barrier to humans. The potential for the creation of new influenza viruses in pigs and the susceptibility of pigs to SIV stipulated our investigations on SIV virulence factors using an experimental SIV infection model in pigs.