Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 9/28/2003
Publication Date: N/A
Technical Abstract: Mycobacterium avium subsp. paratuberculosis (Map) is a slow-growing bacterium that is the causative agent of bovine Johne's disease and also implicated as an etiologic agent of Crohn's disease in humans. The complete genome sequence of the K-10 strain of Map was determined in order to enable a better understanding of the natural physiology and genetic basis of virulence of this important pathogen. The results of our analyses show that the Map genome comprises of 4,829,783 base pairs with G+C content of 69.3% and encodes a total of 4,344 genes. The analysis identified over 3,000 genes with homologues in M. tuberculosis and 702 genomic islands that are unique to Map. Preliminary studies show that the genes encoded in these unique regions are likely to enable the development of new generations of diagnostic tests and vaccines for bovine Johne's disease. Ongoing investigations suggest that the availability of the complete genomic sequence will provide a strong foundation to study the genetic basis for virulence and host-specificity in Map.