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United States Department of Agriculture

Agricultural Research Service


item Wolfenden, A
item Pixley, C
item Johonson, D
item Higgins, Stacy
item Bielke, Lisa
item Nava, G
item Tellez, G
item Donoghue, Dan
item Donoghue, Ann - Annie
item Hargis, Billy

Submitted to: Food Safety Consortium Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 9/30/2003
Publication Date: 10/7/2003
Citation: Wolfenden, A.D., Pixley, C.M., Johonson, D.A., Higgins, S.E., Bielke, L.R., Nava, G.M., Tellez, G.I., Donoghue, D.J., Donoghue, A.M., Hargis, B.M. 2003. Evaluation of a probiotic consisting of nine non-pathogenic bacteria for prophylactic treatment of salmonella enteritidis in broiler chicks. [CD-ROM]. Food Safety Consortium Proceedings.

Interpretive Summary:

Technical Abstract: Previously, we have demonstrated that a simple probiotic consisting of 9 air-tolerant bacteria, could prevent Salmonella enteritidis (SE) infection in turkey poults. Presently, these 7 Enterobacteriaceae and 2 lactic acid bacteria were combined into a single culture and were tested for prophylactic ability to inhibit SE colonization in neonatal broiler chicks. In exp 1, day-of-hatch chicks were randomly divided into five pens (n=20/pen). All chicks were orally gavaged and 3 pens were treated with probiotic culture in the drinking water for four consecutive days. Controls were gavaged with 0.9% sterile saline solution and received no probiotic in the drinking water. Group B received the highest dose of 2.9 x 105 cfu by oral gavage and 1.20 x 107 cfu/ml in the drinking water. Groups C and D received serial 100-fold dilutions of the highest dose. All chicks were challenged 48 h after placement with 4.6 x 103 cfu SE. Cecal tonsils were sampled 48 h post-challenge and cultured for presence or absence of SE. We recovered SE from 87% of non-treated control chicks, and from all treatment groups receiving the probiotic SE was recovered with a lower (p<.05) incidence (B=55%, C=20%, D=35%). Group C also had lower (p<.05) incidence of SE recovery than group B. In exp 2, four groups (n=40) of chicks were placed on the day-of-hatch. Two pens were treated by inclusion of the probiotic culture (4.89 x 104 cfu/ml) in the drinking water for three days, and the other two pens received no probiotic in the water (non-treated control). Ten seeder chicks per group were challenged with 1.25 x 105 cfu SE on the day-of-hatch, and placed in each of the treatment groups 24 h later. On day eight, liver-spleen and cecal tonsil samples were collected from the 10 seeder chicks and 20 contact chicks in each group. SE was recovered in the cecal tonsils with a lower (p<.05) incidence from the groups that received the culture in the drinking water (32%) as compared to controls (82%). These data suggest that a relatively simple and defined probiotic culture can reduce SE infection in neonatal chicks.

Last Modified: 10/17/2017
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