Submitted to: Animal Genetics
Publication Type: Peer reviewed journal
Publication Acceptance Date: 11/18/2003
Publication Date: 4/2/2004
Citation: Ernst, C., Raney, N., Rilington, V., Rohrer, G.A., Brouillette, J., Venta, P. 2004. Mapping of the FES and FURIN genes to porcine chromosome 7. Animal Genetics. 35:142-143. Interpretive Summary: Two genes feline sarcoma oncogene (FES) and paired basic amino acid cleaving enzyme gene (FURIN) were mapped in the genome of the pig. In the human genome, FURIN and FES are located adjacent to one another on human chromosome 15q26. These genes were expected to map to a segment of pig chromosome 7 containing several QTL associated with body composition. Both genes mapped to the pig chromosome 7 within the confidence interval of the body composition QTL. Localizing these genes in the pig genome improves the human-pig comparative map thus facilitating identification of positional candidate genes to study which affect fat deposition.
Technical Abstract: Fragment of the porcine feline sarcoma oncogene (FES) gene and paired basic amino acid cleaving enzyme gene (FURIN) were amplified and terminally sequenced. Their identities were confirmed by comparison with sequences in the NCBI databases using BLAST. Both amplicons were screened for single nucleotide polymorphisms (SNPs). No SNPs were detected in the FURIN product, but three SNPs including one located in a recognition site for the restriction endonuclease HinfI were identified in the FES product. RFLP analysis of FES in F1 individuals from the MARC Swine Reference Population revealed three alleles segregating generating 100 informative meioses. The FES gene was mapped to chromosome 7 position 82 cM. Radiation hybrid mapping of FES and FURIN was performed using the INRA-University of Minnesota 7,000 rads radiation hybrid panel (IMpRH). FES and FURIN were significantly linked to each other, and the marker from the existing RH map that was most significantly linked to both genes was S0066 (FES LOD = 6.79; FURIN LOD = 6.13) indicating that FES and FURIN map to the centromeric region of SSC7. In the human genome, FURIN and FES are located adjacent to one another on the distal q arm of human chromosome 15 (HSA15) in the cytogenetic band q26.1 Genes located in this region are conserved on the proximal q arm of SSC7. Thus, localization of FES and FURIN to SSC7 agrees with the expected comparative map locations for these genes. In addition, FES and FURIN map within the confidence intervals of several putative QTL for pig fatness traits, and mapping of FES and FURIN to this region improves the human-pig comparative map for HSA15 and SSC7 thus facilitating identification of positional candidate genes.