Submitted to: International Society of Citriculture Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 7/15/2003
Publication Date: 2/15/2004
Citation: Rangel, B., Krueger, R. 2004. Stubborn: genetic diversity of spiroplasma citri in california. International Society of Citriculture Abstracts, p. 95 Interpretive Summary:
Technical Abstract: Citrus stubborn caused disease, by Spiroplasma citri, has caused economical losses to the citrus industry. Current detection methods are labor intensive and are not consistent. As part of a project is to improve the detection methods for Spiroplasma, we are assessing the genetic diversity of isolates from different locations throughout California. We have used molecular techniques such as sequencing, RFLP, ITS, and AFLPs. Due to the reduced genome of the pathogen we have observed low levels of genetic diversity with the exception of AFLPs. We have selected the isolates with genetic differences and maintained them in vivo for further genetic analysis. In addition to assessing the genetic differences, we have sequenced those unique fragments of particular interest to the isolates. This information should increase the knowledge of genetic diversity in S. citri as well as show the broad applicability of the detection methods across isolate types.