Submitted to: Theoretical and Applied Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 7/15/2003
Publication Date: 2/15/2004
Citation: Ghislain, M., Spooner, D.M., Rodriguez, F., Villamon, F., Nunez, J., Vasquez, C., Waugh, R., Bonierbale, M. 2004. Selection of highly informative and user-friendly microsatelites (SSRs) for genotyping of cultivated potato. Theoretical and Applied Genetics. 108:881-890. Interpretive Summary: SSRs, also known as microsatellites, are molecular or DNA tools that are useful to determine similarities and differences of plants and animals. SSRs are analogous to the well-known DNA fingerprinting techniques that are so useful in human forensics. This paper reports the selection of a set of 18 SSRs to distinguish cultivated potatoes. These potato SSRs can be used to distinguish different varieties of cultivated potatoes and can be used to group related potatoes together. We selected these 18 potato SSRs from a much larger collection of 156 SSRs, based on ease of obtaining results, on their ability to tell potatoes apart the best, and their distribution on all 12 potato chromosomes. This study is significant because it identifies very useful SSR tools to other potato scientists, and will increase the efficiency of their research using SSRs.
Technical Abstract: Characterization of nearly 1000 cultivated potato accessions with simple sequence repeats (SSRs; also referred to as microsatellites) has allowed the identification of a reference set of SSR markers for accurate and efficient genotyping. A first criterion was ubiquity of the SSRs, most of which were found in DNA sequences of advanced cultivars and the eight landrace cultivar groups of potato, Solanum tuberosum. All SSRs tested in the present study displayed the same allele phenotype and allele size range in the diverse germplasm set as the advanced potato cultivar germplasm from which they were originally developed. Thirteen of 13 SSR products from all cultivar groups have been shown to cross-hybridize with excised and labeled bands of their respective advanced potato cultivar amplification product. Other important SSR selection criteria are quality of amplification products, locus complexity, polymorphic index content (PIC), and well-dispersed location on a potato genetic map. Screening of 156 SSRs has allowed us to identify an efficient and maximally informative set comprising 18 SSR markers for use in characterization of potato genetic resources. In addition, we have identified true- and pseudo-multiplexing SSRs for even greater efficiency. New map locations of 31 SSRs are reported here on an inter-specific potato genetic map.