Submitted to: Annual Conference on Functional Genomics
Publication Type: Abstract Only
Publication Acceptance Date: 5/9/2003
Publication Date: 5/9/2003
Citation: NEILL, J.D., RIDPATH, J.F. REMODELING OF CELLULAR FUNCTIONS FOLLOWING INFECTION WITH BOVINE VIRAL DIARRHEA VIRUS TYPE 2 AS DETERMINED USING SERIAL ANALYSIS OF GENE EXPRESSION. INTERNATIONAL SYMPOSIUM ON ANIMAL FUNCTIONAL GENOMICS. 2003. AVAILABLE FROM: http://www.isafg.msu.edu/posters.html. Interpretive Summary:
Technical Abstract: Bovine viral diarrhea virus (BVDV) exists as one of two biotypes, BVDV type 1 or BVDV type 2. The most serious forms of disease caused by BVDV, severe acute and severe acute hemorrhagic syndrome, are caused by a limited number of BVDV type 2 strains. The determinants of virulence are unknown. To investigate virus-host interactions of the BVDV2 infection, serial analysis of gene expression (SAGE), a powerful sequence-based method to measure global gene expression, was employed to compare gene expression levels in noninfected and in cells infected with the virulent noncytopathic BVDV2 strain 1373. Comparison of the two libraries revealed a number of genes with altered transcription levels that could be placed into functional categories reflecting changes in the biochemical processes of BVDV2-infected cells. Decreases in transcription were noted for genes that participate in cap-dependent translation initiation, including eIF1, eIF4a and eIF4e. Transcripts of genes necessary for translation of the BVDV polyprotein showed small or no changes. At the same time, expression of genes involved in protein translation and post-translational processing were generally upregulated. These included transcripts encoding ribosomal proteins, translation elongation factor 2, tRNA synthases and proteins that interact with nascent polypeptides during transport into the endoplasmic reticulum (Sec61a and translocating chain-associating membrane protein (TRAM)). Additionally, transcripts encoding proteins that participate in vesicle movement and targeting were more abundant in BVDV2-infected cells, including endobrevin, and soluble NSF attachment protein a (SNAPa). These findings indicated that BVDV2 infection results in increased transcription of genes that result in remodeling of the cell to increase efficiency of cellular functions necessary for viral replication, maturation and release.