Submitted to: Plant and Animal Genome
Publication Type: Abstract Only
Publication Acceptance Date: 1/4/1996
Publication Date: N/A
Citation: Reddy, A.S., Haisler, R.M., Weller, J.W., Yu, J., Kohel, R.J. 1996. Development of amplified fragment length polymorphic markers in cotton [abstract]. Plant Genome IV Conference. Paper No. P74.
Technical Abstract: Amplified Fragment Length Polymorphism (AFLP), developed by Keygene, is a new technique geared to generate molecular markers rapidly using either fluorescent or radioactive label. The goal of our project is to develop DNA markers for marker-assisted cotton breeding. The population we have chosen for this project is an F2 population from an interspecific cross between Gossypium hirsutum acc. TM-1 and G. barbadense acc. 3-79. These two parents are useful for the mapping of agronomic traits such as high quality fiber characteristics of 3-79 and the high productivity of TM-1. Genomic DNAs from the two parents and 94 F2 population were isolated and processed for AFLPs using Applied Biosystems AFLP plant mapping kit. The amplified products were then mixed with fluorescent-labled internal standard DNA marker and analyzed on the ABI373 automated DNA sequencer. The AFLP image data was analyzed by the GENESCAN 672 software program. Three to ten AFLPs are detected for each primer pair tested. We are testing 64 primer combinations and expect to have approximately 300 AFLP markers in cotton. Our results indicate that it is feasible to find AFLPs in the complex genomes like cotton and er anticipate that this marker technology will facilitate the progress of cotton genome mapping projects.