Submitted to: Genetic Control of Cotton Fiber and Seed Quality Workshop
Publication Type: Proceedings
Publication Acceptance Date: 12/6/2000
Publication Date: 12/6/2000
Citation: KOHEL, R.J., YU, J., DECANINI, L.I., DONG, J., ZHANG, H. COTTONSEED QUALITY AND CREATION OF GLANDING PLANTS WITH GLANDLESS SEED. PROCEEDINGS OF GENETIC CONTROL OF COTTON FIBER AND SEED QUALITY WORKSHOP. 2000. P. 271-277. Interpretive Summary: Cottonseed contains gossypol and other toxic compounds that limit its use as one of the highest quality oilseeds. Reduction or elimination of gossypol in the cottonseed is the emphasis of cottonseed quality improvement programs. This invited paper reviewed research on cottonseed quality with different approaches. Our research in this area is to totally remove the cottonseed glands that have gossypol and other toxic compounds while retaining them in other parts of the cotton plant for pest resistance. We produced a pair of cotton lines with or without a single gene for gland production. We identified DNA markers that were linked to this gene, and we constructed a library of large DNA clones that contained the gene. With these molecular tools and information, we discussed how to isolate this gene to engineer glanded cottons with glandless seed.
Technical Abstract: We discussed the background of research on cottonseed quality, and we reviewed our research on cottonseed quality. The emphasis of improving cottonseed quality is the reduction or elimination or gossypol in the seed. The research we have conducted the glandless genes form the basis on which plan to create glanded plants that produce glandless seed. The near isogenic line for the gene Gl2e is foundation for our research. Research which has identified DNA markers linked to the Gl2 locus, and the construction of a BAC library. We report the progress of our research, and we outline our research plans for cloning the Gl2e gene and using it to engineer the glanded plants that produce glandless seed.