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United States Department of Agriculture

Agricultural Research Service

Title: {U-13C]GLUCOSE MIDA PROVIDES ACCURATE MEASURES OF GLUCONEOGENESIS, IS EASY TO PERFORM AND REQUIRES ONLY SMALL BLOOD SAMPLE VOLUMES)

Author
item Sunehag, Agneta
item Clarke, Lucinda
item Bier, Dennis
item Haymond, Morey

Submitted to: Diabetes
Publication Type: Abstract Only
Publication Acceptance Date: 4/1/2001
Publication Date: 6/1/2001
Citation: SUNEHAG, A., CLARKE, L., BIER, D.M., HAYMOND, M. {U-13C]GLUCOSE MIDA PROVIDES ACCURATE MEASURES OF GLUCONEOGENESIS, IS EASY TO PERFORM AND REQUIRES ONLY SMALL BLOOD SAMPLE VOLUMES. DIABETES. 2001.

Interpretive Summary: Not required.

Technical Abstract: Background: The validity of recent in vivo methods to measure gluconeogenesis has been debated. The deuteriumoxide method with measurements of deuterium enrichment at glucose C5 is a straightforward approach to measure gluconeogenesis but is very time consuming and requires blood sample volumes which preclude its use in infants and children. The present study was undertaken to compare [U-13C]glucose MIDA, which is easy to perform and requires very small sample volumes, with that of the deuterium oxide glucose C5 method. Methods: 5 healthy male adults (age 28±3 y, weight 71±4 kg, BMI 23±1 kg/m2) (mean ± SE) were studied on two occasions. On each occasion, 8 h tracer infusions were begun 58 h into the fast. On one occasion, gluconeogenesis and glucose production were measured by [U-13C]glucose (study I), and on the other gluconeogenesis was estimated by the deuterium oxide C5 method and glucose production by [6,6-2H2]glucose (study II). Deuterium oxide (a total of 3 g/kg) was administered as 4 oral doses given at 2 h intervals beginning 48 h into the fast. Results: All data represent mean±SE of 5 measurements obtained between study h 7 and 8 (fasting h 64-65). No differences were observed between the two study occasions in plasma glucose, 4.0±0.2 (study I) vs. 4.0± 0.2 mM(study II) (ns), plasma glucose appearance rates, 6.8± 0.3(study I) and 6.9±0.2 micromol/kg min (study II) (ns) or glucose production rates, 5.8±0.3 (study I) and 6.0±0.3 micromol/kg min (study II) (ns). The estimates of gluconeogenesis provided by the two methods did also not differ significantly. Thus, the gluconeogenic rate measures by {U-13C]glucose MIDA was 5.1±0.2 and by deuteriumoxide glucose C5, 5.7±0.3 micromol/kg min (ns) i.e. gluconeogenesis accounted for 87±2 and 96±5% of GPR, respectively (ns). Conclusion: The [U-13C]glucose MIDA and the deuteriumoxide glucose C5 method provided similar estimates of gluconeogenesis. Additionally, the [U-13C]glucose measurements are highly reproducible. Thus, [U-13C]glucose MIDA provides a robust approach to measure gluconeogenesis, which can be easily and safely applied to all groups of subjects including very premature infants.

Last Modified: 8/24/2016
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