Submitted to: Animal Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 12/6/2002
Publication Date: 4/20/2003
Citation: KIM, J.G., NONNEMAN, D.J., VALLET, J.L., CHRISTENSON, R.K. BRIEF NOTE. LINKAGE MAPPING OF THE PORCINE TESTIS ENHANCED GENE TRANSCRIPT (TEGT) GENE TO CHROMOSOME 5. ANIMAL GENETICS. 2003. v. 34(2). p. 152-153. Interpretive Summary: Sperm production is an important reproductive trait for swine industry. The testis enhanced gene transcript (TEGT) gene has been known to support the survival of mammalian cells. This gene is highly expressed in adult rat testis, and thus it may support the survival of germ cells or support cells in the testis, which may lead to increased sperm production. However, TEGT cDNA sequence or the chromosomal location of the gene in the pig is not known. As a first step to determine whether the TEGT gene influence male reproductive function, especially sperm production, a cDNA clone containing a partial coding region of the TEGT (GenBank accession no. AY166682) was isolated from the "Meat Animal Research Center (MARC) 2PIG" expressed sequence tag (EST) library and the TEGT gene was mapped to chromosome 5 position 52 cM.
Technical Abstract: A cDNA clone containing the partial coding region of the porcine testis enhanced gene transcript (TEGT) was isolated (GenBank accession no. AY166682) from the "Meat Animal Research Center (MARC) 2PIG" expressed sequence tag (EST) primary library by iterative screening and sequenced. The carboxy terminal 15 amino acids derived from the partial porcine TEGT cDNA are identical with the carboxy terminal 15 amino acids of the human TEGT (GenBank accession no. NM_003217) and the 3' untranslated region of the porcine TEGT shares 75% identity with the human TEGT. The forward (TEGT F1) and reverse (TEGT R1) primers that were used for PCR amplification correspond to bases 6-24 and 374-356 of the porcine cDNA sequence (GenBank accession no. AY166682), respectively. The forward (TEGT F2) and reverse (TEGT R3) primers that were designed to amplify a 927 bp product in the 3' untranslated region of the cDNA correspond to bases 501-520 and 1445-1426 of the porcine TEGT cDNA, respectively. A G/T single nucleotide polymorphism was detected at position 715 of the porcine TEGT cDNA (GenBank accession no. AY166682). This polymorphism was heterozygous in 2 of the 7 F1 sows. An assay was designed to genotype this polymorphism using primers TEGT F2 and R3, primer extension with the TEGT probe primer, and analyte detection on a MALDI-TOF mass spectrometer with 50 ul PCR reactions. PCR conditions were same as above. This marker generated 29 informative meioses in the MARC swine reference population. The TEGT gene was mapped to chromosome 5 position 52 cM, which is the same position as marker SWR453 on the current MARC swine chromosome 5 linkage map (http://www.marc.usda.gov/) using CRI-MAP. The most significant two-point linkage detected was with SWR453 (LOD = 6.62) at 0 recombination. The TEGT gene in human is located on chromosome 12q12-q13, which shares homology with swine chromosome 5. TEGT was identified as Bax inhibitor-1 in mammalian cells, which functions as a suppressor of apoptosis. The transcript of TEGT is abundant in adult rat testis and may support the survival of certain cell types in the testis.