Submitted to: Research Workers in Animal Diseases Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 11/15/2002
Publication Date: N/A
Citation: N/A Interpretive Summary:
Technical Abstract: Unmethylated CpG dinucleotides in particular base contents that are relatively abundant in bacterial DNA are known to stimulate innate immune responses including immune cell proliferation and cytokine secretion in mammalian. Here, we have screened more than 20 CpG-ODN for their ability to stimulate nitric oxide (NO), IL-1beta, and IFN-gamma production using chicken macrophage cell HD-11 and peripheral blood mononuclear cells. Our results showed that synthetic oligodeoxynucleotides containing CpG motif were able to activate the avian macrophage cells and induce NO production. The optimal CpG-ODN motif for NO induction was GTCGTT. Increase of the number of GTCGTT motifs in the CpG-ODN significantly enhanced the stimulatory effect. Deviation of one or two flanking bases on the CpG dinucleotide reduced or diminished the stimulatory activity. We also found that CpG-ODN differentially stimulated expression of cytokine genes. The optimal CpG motif for NO induction was also a strong stimulant for the IL-1beta gene expression in the macrophage HD-11 cells, whereas different CpG motifs have found to induce the IFN-gamma gene expression in PMNC.