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United States Department of Agriculture

Agricultural Research Service

Title: ABILITY OF AN ELISA-BASED SEED HEALTH TEST TO DETECT ERWINIA STEWARTII IN MAIZE SEED TREATED WITH FUNGICIDES AND INSECTICIDES)

Author
item Michener, P
item Pataky, J
item Block, Charles
item Shepherd, L
item Mcgee, D

Submitted to: American Phytopathological Society Annual Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 7/31/2002
Publication Date: 7/31/2002
Citation: Michener, P.M., Pataky, J.K., Block, C.C., Shepherd, L.M., Mcgee, D.C. 2002. Ability of an elisa-based seed health test to detect erwinia stewartii in maize seed treated with fungicides and insecticides [abstract]. Phytopathology. 92:S56

Interpretive Summary:

Technical Abstract: An ELISA-based seed health test detected E. stewartii in infected maize seed treated with 11 combinations of captan, carboxin, clothianidin, imidacloprid, thiamethoxam, fludioxonil, mefenoxam, or thiram. Treated, healthy seeds were blended with treated, E. stewartii-infected seeds of Jubilee or A632 to produce 100-kernel samples with a 96% or 88% chance, respectively, that a randomly-selected sample would contain infected kernels. Forty-six samples were tested per seed treatment and a non-treated control. Based on Type I errors near 5%, >20 of 23 samples per treatment were expected to be positive for samples with Jubilee, and >17 of 23 samples per treatment were expected to be positive for samples with A632. For Jubilee, all treatments had >20 positive samples. For A632, all treatments had >17 positive samples except for seed treated with clothianidin or captan/carboxin which had 17 and 15 positive samples, respectively. Mean absorbance values of positive samples did not differ among seed treatments.

Last Modified: 8/24/2016
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