Submitted to: Animal Genetics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 11/5/2001
Publication Date: 4/1/2002
Citation: Knoll, A., Dvorak, J., Rohrer, G.A., Cepica, S. 2002. Brief note. Linkage and cytogenetic mapping of the BCL9 gene to porcine chromosome 4. Animal Genetics. 33:162-163.
Interpretive Summary: A gene which affects fat metabolism in humans (B-cell CLL/Lymphoma 9; BCL9) was mapped in the pig as a candidate gene for body composition. This gene was placed on pig chromosome 4 in the same region where a QTL for fatness has previously been mapped. Additional studies are needed to determine if this gene affects body composition in pigs.
Technical Abstract: A fragment of the porcine BCL9 (B-cell CLL/Lymphoma 9) gene encompassing parts of exons 6 and 7 and intervening intron was amplified using primers from cDNA sequence of the human gene (NM_004326) compared with the human chromosome 1 working draft sequence segment NT_004434. To verify the identity of the amplicon with an approximate length of 1.9 kb, the fragment was cloned and terminally sequenced. The sequence data obtained have been deposited in the EMBL database (accession numbers AJ416470 and AJ416471) and compared with sequences in NCBI databases using BLAST2. The sequences AJ416470 and AJ416471 matched exons 6 and 7 of the human cDNA sequence (NM_004326) with identities of 100% (54/54) and 96% (375/390), respectively. A polymorphism was detected and used to place BCL9 on porcine chromosome 4, position 81 on the MARC swine genetic map.