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ARS Home » Plains Area » Grand Forks, North Dakota » Grand Forks Human Nutrition Research Center » Dietary Prevention of Obesity-related Disease Research » Research » Publications at this Location » Publication #136532
Title: EFFECT OF SELENIUM-ENRICHED BROCCOLI DIET ON DIFFERENTIAL GENE EXPRESSION IN MIN MICE LIVER

Author
item ZENG, HUAWEI - 5450-20-00
item DAVIS, CINDY - 5450-20-00
item FINLEY, JOHN - 5450-20-00

Submitted to: Journal of Nutritional Biochemistry
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 12/20/2002
Publication Date: 4/1/2003
Citation: Zeng, H., Davis, C.D., Finley, J.W. 2003. Effect of selenium-enriched broccoli diet on differential gene expression in min mouse liver. Journal of Nutritional Biochemistry 14:227-231.

Interpretive Summary: The essential nutritional role of selenium (Se) in animals and humans is well established. Previous studies have shown that selenium-enriched broccoli reduces colon cancer susceptibility effectively. Because colon cancer cells mainly metastasize to the liver, the study of effects of selenium-enriched broccoli on cancer related gene expression in the liver was undertaken to further our understanding of the protective role for selenium-enriched broccoli in tumorigenesis. This study investigated the effects of selenium-enriched broccoli on the binding of important liver transcription factors to their cis-acting elements, which is the essential step in the control of gene expression. Our results suggest that selenium-enriched broccoli enhances the binding of numerous transcription factors particularly p53 (a tumor suppressor gene product) to cis-acting elements. The data may extend the understanding of the anti-cancer effect of selenium-enriched broccoli. These findings will be useful for scientists and health-care people who study food and cancer prevention.

Technical Abstract: Previous studies have shown that selenium-enriched broccoli effectively reduces colon cancer susceptibility. Because colon cancer cells mainly metastasize to the liver, studying the effects of selenium-enriched broccoli on cancer-related gene expression in the liver might increase our understanding of the protective role for selenium-enriched broccoli in tumorigenesis. This study determined the effects of selenium-enriched broccoli on the binding of important liver transcription factors to their cis-acting elements, which is essential in the control of gene expression. Our results suggest that selenium-enriched broccoli enhances the binding of numerous transcription factors particularly p53 protein (a tumor-suppressive transcription factor) to cis-acting elements. These observations may have additional implication for understanding of an anti-cancer effect of selenium-enriched broccoli.