Submitted to: Meeting Abstract
Publication Type: Abstract only
Publication Acceptance Date: 3/20/2002
Publication Date: 4/17/2002
Citation: Parkin, K., Wettasinghe, M., Bolling, B., Xiao, H., Peterson, D.M., Plhak, L. 2002. Methodological considerations for the murine hepatoma cell bioassay-directed isolation of cancer chemopreventive agents. Meeting Abstract. Interpretive Summary:
Technical Abstract: The development of a murine hepatoma (Hepa 1c1c7) cell line over a decade ago for the isolation of phase II enzyme inducing agents by a group from Johns Hopkins University led to the identification of sulforaphane as a major cancer chemopreventive agent in broccoli. We have found that choices among alternative methodological considerations using this bioassay can have profound impact on the ability to detect and isolate phyase II enzyme inducing agents from botanical sources. These considerations include the nature of raw material preparation, the specific solvent used to extract the prospective bioactive agent(s) and the inclusion of even minor amounts of nonaqueous solvent as carrier of the agent or extract into the bioassay. The impact of these choices and consideration on the discovery and documentation of the phase II inducing effect of extracts of known compounds will be illustrated using examples from the authors' laboratories involving the study of common vegetables and grains.