Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 12/6/2000
Publication Date: 1/11/2001
Technical Abstract: The USDA-ARS cotton genome research program at College Station, Texas, consists of several on-going projects including (1)integrative genetic and physical mapping;(2)genomic localization of major genes and QTLs;(3)molecular characterization of Gossypium germplasm;(4)bridging cotton with plant model systems; and(5)development and curation of CottonDB. A molecular map(>1,000 DNA markers) of the cotton genome was developed from a cross between G. hirsutum L. acc. TM-1 and G. barbadense L. acc. 3-79, and recombinant inbred mapping population was made available for consensus mapping efforts. Twenty QTLs for fiber quality and genes for fiber and seed quality were located in the cotton genome. A codominant allele (Gl2**e) is targeted for its positional cloning to create glanded cotton plants with glandless cottonseeds. Six BAC and BIBAC libraries were constructed from TM-1, the genetic standard of G. hirsutum, and its glandless isoline ESP. While ESP libraries consist of 115,584 clones, TM-1 libraries consist of 172,800 clones. New SSR markers were isolated from the positive TM-1 BAC clones to facilitate integrative genetic and physical mapping of the cotton genome. DNA profiles of the 280 U.S. and Russian Gossypium accessions were generated with an initial set of 100 selected core DNA markers. Molecular characterization will be expanded to accessions(~13,000)in the National Collection of Gossypium germplasm maintained by USDA-ARS, in College Station, Texas. A preliminary survey indicates that 10% of 40,000 screened Arabidopsis ESTs are readily applicable in cotton. Detailed analysis of this portion of Arabidopsis ESTs will greatly facilitate cotton improvement programs. Genomic data and information related to Gossypium spp. continue to be curated into CottonDB, the National Plant Genome Database for cotton.