Submitted to: Eastern Wheat Workers and Southern Small Grain Workers Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 5/1/1996
Publication Date: N/A
Technical Abstract: Genes for Hessian fly resistance have been used in wheat to help prevent much of the crop loss this pest can cause. Recently, breeders have begun trying to place multiple genes for Hessian fly resistance into a single cultivar, hoping to increase its durability in the field. To this end, five nearly isogenic wheat lines were constructed, each containing a single new locus for Hessian fly resistance (HFR). These 5 HFR loci were tested against four different fly populations and were shown to have high levels of resistance to each one. Bulks of ten plants from each of these five lines plus a susceptible cultivar, Cardinal, were screened with approximately 600 RAPD primers. From these initial screens, 40 of the primers detected usable polymorphisms. From this subset, five primers retained the initial polymorphism when individual plants (not bulks) were tested. With these five primers, 4 out of the 5 loci have putative markers which will be used as an aid in the gene stacking process.