|De Los Reyes, Benildo|
|Mcgrath, J Mitchell - Mitch|
Submitted to: National American Phytopathology Meetings
Publication Type: Abstract Only
Publication Acceptance Date: 8/27/2001
Publication Date: N/A
Technical Abstract: Rhizoctonia crown and root rot is a serious disease of sugar beets in the US. Warm weather of mid-summer favors the development of rot, but by mid- August, the rotting of tissue ceases and the infected tissue is restricted and demarcated from healthy tissues. Defense-related phenolic compounds are produced at the boundary between infected and healthy tissues. Our previous study showed that the induced production of chitinases during defense is a generalized wound response. To further investigate the plant defense, we developed a subtractive, disease response-specific cDNA library from infected roots using mRNA derived from disease-free roots as driver for the substraction. 768 clones were recovered after two rounds of substraction via the biotin-avidin method. The library then was screened for chitinase-related sequences using a probe developed from an Arabidopsis acidic endochitinase gene (M34107. Using RT-PCR, the cDNAs isolated are currently being used to investigate pathogen-induced chitinase expression at the transcriptional level. Additionally, this library will be used as a source of defense response specific, expressed sequence tags.