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ARS Home » Midwest Area » East Lansing, Michigan » Sugarbeet and Bean Research » Research » Publications at this Location » Publication #125616

Title: MOLECULAR AND BIOCHEMICAL ANALYSIS OF THE DEFENSE RESPONSE OF SUGARBEET TAP ROOTS TO INFECTION BY RHIZOCTONIA SOLANI

Author
item Nagendran, Subashini
item De Los Reyes, Benildo
item Halloin, John
item Mcgrath, J Mitchell - Mitch

Submitted to: National American Phytopathology Meetings
Publication Type: Abstract Only
Publication Acceptance Date: 8/27/2001
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Rhizoctonia crown and root rot is a serious disease of sugar beets in the US. Warm weather of mid-summer favors the development of rot, but by mid- August, the rotting of tissue ceases and the infected tissue is restricted and demarcated from healthy tissues. Defense-related phenolic compounds are produced at the boundary between infected and healthy tissues. Our previous study showed that the induced production of chitinases during defense is a generalized wound response. To further investigate the plant defense, we developed a subtractive, disease response-specific cDNA library from infected roots using mRNA derived from disease-free roots as driver for the substraction. 768 clones were recovered after two rounds of substraction via the biotin-avidin method. The library then was screened for chitinase-related sequences using a probe developed from an Arabidopsis acidic endochitinase gene (M34107. Using RT-PCR, the cDNAs isolated are currently being used to investigate pathogen-induced chitinase expression at the transcriptional level. Additionally, this library will be used as a source of defense response specific, expressed sequence tags.