Submitted to: Crop Science
Publication Type: Abstract Only
Publication Acceptance Date: 5/1/1996
Publication Date: N/A
Citation: Morris, J.B., Hopkins, M.S. Legume genetic resources for special-purpose nutraceutical and pharmaceutical use. Crop science, 1996. Agronomy abstracts 88:175.
Technical Abstract: Tissue culture regeneration of "Crotalaria, Indigofera, Lablab purpureus, Senna and Sesbania" species explants from deteriorated seed fo these leguminous species were incubated at an 8h photoperiod at 26C on MSB5 medium containing MS salts, B5 vitamins, 20 g/L sucrose, and 8 g/L agar. Three-wk old plants regenerated from seed were transplanted to soil inside the greenhouse. Thirty-nine samples of deteriorated seed between 2 and 47 yr-old were evaluated. Significant differences in organogenesis were observed between different seed accessions. Shoots and roots were recovered from 70% and 83% of seed explants. The in vitro rescue of seed can significantly increase the recovery of genetic resources from deteriorated seed of these leguminous species.