Submitted to: Animal Genetics
Publication Type: Peer reviewed journal
Publication Acceptance Date: 8/7/2000
Publication Date: N/A
Citation: Interpretive Summary: A procedure to isolate microsatellite genetic markers from dissected chromosomal DNA was developed. To demonstrate this procedures' utility, a library was produced which was expected to contain segments of porcine chromosome 13. Five microsatellite markers were developed and mapped in the US-MARC swine reference population. Three of the five markers mapped to chromosome 13 while one marker mapped to chromosome 15 and one to chromosome 16. The procedure was successful in targeting a specific chromosome, but the library was not exclusive to one porcine chromosome.
Technical Abstract: We successfully modified a microsatellite enrichment method developed for genomic libraries for DOP-PCR amplified microdissected DNA. With this approach we isolated three informative microsatellite markers from chromosome 13. In addition, one informative microsatellite mapped to chromosome 15 and one to chromosome 16. The most likely explanation for markers located on chromosomes other than 13 is that these chromosomes were inadvertently contacted by the microdissection needle, thus contaminating the sample. These additional markers, while not mapping to the desire region, are still valuable since they add informative markers to the genetic map in other regions of the genome. Chromosome 13 is the second largest porcine chromosome. A putative QTL associated with early growth (birth to 30 kg) make chromosome 13 a priority chromosome in efforts to map the pig genome, and this QTL appears to influence birth weight.