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ARS Home » Midwest Area » Ames, Iowa » National Animal Disease Center » Virus and Prion Research » Research » Publications at this Location » Publication #117061
Title: THE USE OF PORCINE ALVEOLAR MACROPHAGES FOR DETECTING SHIGA TOXIN

Author
item Mengeling, William
item Vorwald, Ann
item CORNICK, N - IOWA STATE UNIVERSITY
item Lager, Kelly
item MOON, H - IOWA STATE UNIVERSITY

Submitted to: American Association of Veterinary Laboratory Diagnosticians
Publication Type: Abstract Only
Publication Acceptance Date: 10/1/2000
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Porcine alveolar macrophages were found to be highly susceptible to the cytolytic effects of a toxin (Shiga toxin [Stx]) produced by certain strains of Escherichia coli and sometimes associated with clinical disease in pigs and other animals. In comparison with the cells that are most commonly used for Stx detection (namely, Vero cells) porcine alveolar macrophages were found to be generally more sensitive, and test results could be obtained in less time. Moreover, unlike continuous cell lines such as Vero cells, porcine alveolar macrophages need not be continuously propagated to ensure almost instant availability. They can simply be removed from a low-temperature repository, thawed, seeded, and exposed to test sample shortly thereafter. These advantages suggest that porcine alveolar macrophages should be evaluated as a sensitive and timely diagnostic test for Stx.