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ARS Home » Midwest Area » Ames, Iowa » National Animal Disease Center » Food Safety and Enteric Pathogens Research » Research » Publications at this Location » Publication #110772
Title: COMPARISON OF A MULTIPLEX AND 5' NUCLEASE PCR ASSAYS FOR THE RAPID DETECTION OF PATHOGENIC YERSINIA ENTEROCOLITICA IN SWINE AND PORK PRODUCTS (SIM: FOOD-BORNE PATHOGENS 2000, ARLINGTON, VA, 4/16-19/00)

Author
item BOYAPALLE, S - TUSKEGEE UNIVERSITY
item KANUGANTI, S - TUSKEGEE UNIVERSITY
item Wesley, Irene
item Hurd, Howard
item REDDY, P - TUSKEGEE UNIVERSITY

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 4/19/2000
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Pigs are the major animal reservoirs of human pathogenic strains of Yersinia enterocolitica (YE). Our objective was to compare bacteriological culture method with PCR-based protocols (multiplex PCR and TaqMan 5' nuclease PCR) for the rapid detection of pathogenic YE in market weight hogs and pork products. YE prevalence was compared in hog specimens collected before (fecal, tonsil scrapings) and after slaughter (lymph nodes, tonsils, cecal and rectal contents). By bacteriological culture method, YE was not detected in any hog tissues. By multiplex PCR, YE was detected in tonsil scrapings (2%), and tonsils (3.5%). The highest prevalence of YE was in ileocecal lymph nodes both by multiplex PCR (7%) and TaqMan assay (39.9%). TaqMan assay detected YE in ~27% of all hog tissues. Chitterlings and ground pork were also screened for YE. The results suggest (i) YE was more frequently detected in chitterlings and ground pork than in freshly slaughtered hogs; and (ii) TaqMan assay appears to be several-fold more sensitive than multiplex PCR and culture methods.