|SANGTONG, VARAPORN - IOWA STATE UNIVERSITY
|LEE, MIKE - IOWA STATE UNIVERSITY
Submitted to: Maize Genetics Conference Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 3/11/1999
Publication Date: N/A
Technical Abstract: SDS PAGE and western blot analysis were used to characterize expression of the wheat high-molecular weight glutenin gene 1Dx5 in maize. When F1 transgenic plants were crossed with an inbred line (B73), the 1Dx5 subunit was detected in endosperm of progeny only when the transgenic plant was used as a female parent. These progeny had 11 (presenceabsence) ratios of 1Dx5 expression. In addition, most of the F2 transgenic families resulting from self-pollinating F1 transgenic plants had 11 (presenceabsence) ratios of 1Dx5 expression, which fit a model in which the transgene is only transmitted through the female. However, when these plants were crossed as males to B73 females, 2-11 % of the resulting progeny showed 1Dx5 expression. In a preliminary effort to elucidate the mechanism of this inheritance pattern, we examined the pollen of the plants. In vitro germination was lower in transgenic plants than in non-8transgenic plants. Collectively, these data are consistent with the hypothesis that the 1Dx5 transgene inefficiently transmitted through pollen.