Submitted to: Experimental Biology
Publication Type: Abstract Only
Publication Acceptance Date: 4/1/2000
Publication Date: N/A
Interpretive Summary: An interpretive summary is not required.
Technical Abstract: Some fatty acids (FA) influence the differentiation of adipocytes and may be ligands for peroxisome proliferator-activated receptor gamma (PPAR gamma). To determine the effect of FA on transcripts for PPAR gamma, adipocyte differentiation determination factor 1 (ADD1), adipocyte fatty acid binding protein (aP2), and lipoprotein lipase (LPL), porcine stromal vascular cells were differentiated to adipocytes in vitro with or without FA. Addition of 50 to 300 muM oleic acid (C18:1), linoleic acid (C18:2), conjugated linoleic acid (CLA), linolenic acid (C18:3), and arachidonic acid (C20:4) for 1 day significantly increased adipocyte differentiation. Docosahexaenoic acid (C22:6) had no effect on adipocyte differentiation. The mRNA concentrations for PPAR gamma, and LPL were increased by C18:1, C18:2, and C20:4, but not by C18:3 and C22:6. All the unsaturated FA tested significantly increased the aP2 mRNA concentration (two- to seven-fold). Regardless of the effect on differentiation, C20:4, C22:6, an CLA decreased ADD1 mRNA. These results indicate selected FA increase porcine adipocyte differentiation. Also, the regulation of the expression of ADD1, PPAR gamma, and aP2 by FA may be through different mechanisms.