Submitted to: Proceedings of the National Academy of Sciences
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 9/28/1999
Publication Date: N/A
Citation: N/A Interpretive Summary: Genetic transformation of plants often results in multiple copies of the introduced DNA at a single locus. We demonstrated with wheat transformation that four of four multiple-copy loci could be resolved successfully into single-copy transgenes.
Technical Abstract: Genetic transformation of plants often results in multiple copies of the introduced DNA at a single locus. To ensure that only a single copy of a foreign gene resides in the plant genome, we used a strategy based on site-specific recombination. The transformation vector consists of a transgene flanked by recombination sites in an inverted orientation. Regardless of the number of copies integrated between the outermost transgenes, recombination between the outermost sites resolves the integrated molecules into a single copy. An example of this strategy has been demonstrated with wheat transformation, where four of four multiple-copy loci were resolved successfully into single-copy transgenes.