Submitted to: Journal of Federation of American Societies for Experimental Biology
Publication Type: Abstract Only
Publication Acceptance Date: 12/10/1999
Publication Date: 3/15/2000
Citation: Zaslavsky, B.G., Milne, D.B., Nielsen, F.H., Uthus, E.O. 2000. Statistical assessment of copper status in postmenopausal women [abstract]. The Federation of American Societies for Experimental Biology Journal. 14:A221.
Technical Abstract: Fourteen postmenopausal women were fed a mixed Western basal diet that supplied approximately 0.6 mg copper (Cu) per day (2500 kcal intake). After an equilibration period of 35 days in which the basal diet plus up to 0.8 mg of Cu (as Cu sulfate in juice) was fed, there was a low dietary Cu period of 105 days (divided into 5 stages, 3 wk/stage), where only the basal diet was fed (4 women dropped out during this period). During the study, energy intake was adjusted to maintain the body weight to within 2% of admission weight. We analyzed the data by using ANOVA and logistic regression. The results of logistic analysis suggested that balance data were reliable criteria of Cu deprivation (CuD) predicting dietary Cu status with success above 80%. Cu retention decreased markedly the first 6 wk of CuD. Mean corpuscular hemoglobin and mean corpuscular hemoglobin (MCHC) responded to CuD in all deprivation stages; both indices decreased with CuD. LDH decreased 25% from the equilibration value during the last 6 weeks of CuD. Protein content of monocystes (MNP) predicted Cu status with success >90% in all stages. MNP increased 80% in the first 9 wk of CuD. SOD consistently decreased during CuD. Blood clotting factor 8 increased during CuD and was a very reliable index of Cu status. Calcitonin increased during the last 6 wk of CuD. Glucose tolerance was among the best indices of CuD. Thus statistical analysis revealed time dependent but consistent indicators of CuD.