Skip to main content
ARS Home » Midwest Area » Ames, Iowa » National Animal Disease Center » Food Safety and Enteric Pathogens Research » Research » Publications at this Location » Publication #105590
Title: COMPARISON OF A MULTIPLEX AND 5' NUCLEASE PCR ASSAYS FOR THE RAPID DETECTION OF PATHOGENIC YERSINIA ENTEROCOLITICA IN SWINE AND PORK PRODUCTS (SWINE RESEARCH REPORT - 2000)

Author
item BOYAPALLE, SANDHYA - TUSKEGEE UNIV., ALABAMA
item Wesley, Irene
item REDDY, P - TUSKEGEE UNIV., ALABAMA

Submitted to: Swine Research Report
Publication Type: Proceedings
Publication Acceptance Date: 9/24/1999
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Bacteriological culture methods were compared with PCR based protocols (multiplex PCR and the TaqMan assay) for the rapid detection of pathogenic Yersinia enterocolitica (YE) in market weight hogs and pork products. The prevalence of YE was compared in lairaged hogs (n=150) versus hogs transported directly to the farm (n=150). By bacteriological culture, YE was not detected in any of the hog tissues tested but was detected by multiplex PCR and TaqMan assays. We also screened ground pork and chitterlings for the presence of YE. By standard culture, YE was detected in chitterlings (10%). By multiplex PCR, YE was identified in ground pork (40%) and chitterlings (51%). The TaqMan assay identified YE in ground pork (80%) and chitterlings (85%).