Location: Diet, Microbiome and Immunity Research
Project Number: 2032-10700-002-020-S
Project Type: Non-Assistance Cooperative Agreement
Start Date: Sep 23, 2024
End Date: Dec 22, 2027
Objective:
The overall objective of the cooperative agreement is to determine the impact of pulse intake on the antimicrobial resistance genes in the healthy adult consumer's gut. Specific objectives of the third year are to 1) complete a manuscript on the effect of long-term lentil consumption on the abundances of antimicrobial resistance genes in gut microbiomes of consumers in the Lentil Study, and 2) complete the antimicrobial resistance gene analysis in the FEEDMe study.
Approach:
Banked stool samples and dietary data from participants in two different pulse feeding trials will be used to determine the antimicrobial resistance genes in humans. The two studies to be used:
1) Lentil Feeding Study: This completed PCHI-funded trial (#58-3060-9-040) at Montana State University was a single-blind parallel randomized clinical trial that examined the impact of 12-weeks of lentil consumption on postprandial lipid
metabolism and gut microbial composition. Thirty-eight metabolically at-risk males and females (18-70 y), as defined by elevated waist circumference and postprandial triglycerides, completed the 12-w dietary intervention. For the intervention,
subjects were asked to consume 7 prepared mid-day meals each week that were isocaloric but varied in lentil dosage per week: 0 g or 980 g whole green cooked lentils. Individuals in the control group consumed meals containing either turkey
or chicken instead of lentils. Fecal samples from this trial have now been sequenced at the University of California-Davis.
2) FEEDMe Study: The “Fiber Effectively Educates & Directs the Microbiome (FEEDMe) Study” at UC Davis is a single-group open-label pilot study (n = 48, expected) to examine how diverse, commercially available foods rich in arabinose influence the gut microbiome.