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ARS Home » Pacific West Area » Salinas, California » Crop Improvement and Protection Research » Research » Research Project #445666

Research Project: Development of Germplasm Resources and Molecular Breeding Tools to Combat Endemic and Emerging Diseases in US Spinach Production

Location: Crop Improvement and Protection Research

Project Number: 2038-21530-003-018-R
Project Type: Reimbursable Cooperative Agreement

Start Date: Oct 1, 2023
End Date: Sep 14, 2027

1) Characterize and validate molecular markers for resistance to spinach endemic and emerging diseases. 2) Introgress disease resistance into spinach breeding populations and cultivars.

We will conduct a highly integrated collaborative project with genetic studies to identify chromosomal regions associated with important spinach disease (e.g. downy mildew) resistance traits. We will identify quantitative trait loci (QTLs) and integrate such data into meta-analyses of crop performance, perform high resolution genetic analysis of major QTLs to provide molecular markers and identify candidate genes underlying selected QTLs, and analyze sequences of candidate genes across primary and secondary genepools. We will develop and apply robust easy to use molecular markers for marker-assisted selection. We will perform field evaluations of input and output genotypes, develop spinach populations for the identification and mapping of molecular markers linked to disease resistance, study the inheritance of the traits, and transfer these traits to new spinach cultivars through spinach breeding.