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ARS Home » Midwest Area » Columbia, Missouri » Plant Genetics Research » Research » Research Project #445365

Research Project: Negative Effects of Protease Inhibitors On Monogastric Animal Growth Performance: Truth or Myth?

Location: Plant Genetics Research

Project Number: 5070-21000-044-026-R
Project Type: Reimbursable Cooperative Agreement

Start Date: Oct 1, 2023
End Date: Sep 30, 2024

We propose to develop a simple, reliable and inexpensive procedure that can be routinely utilized by soybean processors as a standard method to measure trypsin inhibitor levels.

We recently developed an easy and rapid procedure to measure trypsin inhibitor activity in soybean meal, by modifying a publicly available method. In this proposal, we will be further refining our assay to increase its reliability. Many samples provided by the feed industry will be used for the validation of our new protocol. We will examine the feasibility of measuring the trypsin inhibitor activity in 96-well microplates which will enable high-throughput screening of large number of soy meal samples.