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ARS Home » Midwest Area » Ames, Iowa » National Animal Disease Center » Food Safety and Enteric Pathogens Research » Research » Research Project #443701

Research Project: Revealing Mechanisms for Internal Salmonella Colonization and Persistence in Porcine Lymphoid and Fat Tissues

Location: Food Safety and Enteric Pathogens Research

Project Number: 5030-32000-225-030-T
Project Type: Trust Fund Cooperative Agreement

Start Date: Mar 1, 2023
End Date: Aug 31, 2024

The objectives of the proposed research are 1) identify the cell types harboring Salmonella in pig lymphoid and adipose tissue at various stages of colonization and 2) perform spatial transcriptomics and single-cell RNA-sequencing of Salmonella-positive lymphoid and/or adipose tissues to deeply characterize the cellular response and identify mechanisms of intracellular colonization.

Pigs will be inoculated with a contemporary foodborne isolate of Salmonella enterica and subsequently euthanized for isolation of ileum (Peyers patch), ileo-cecal lymph node, mesenteric lymph node, mesenteric fat, and palatine tonsil. Tissues will be collected for Salmonella enumeration, in situ identification of cells harboring Salmonella, spatial transcriptomics, and single-cell RNA sequencing. Necropsy timepoints will include 1-, 2-, 5-, 8-, 14- and 28-days post-inoculation. Feces and cecal contents will also be collected for Salmonella enumeration.