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Research Project: Genetic Diversity of Impatiens Necrotic Spot Virus (INSV) Isolates Affecting Important Vegetable and Horticultural Crops

Location: Crop Improvement and Protection Research

Project Number: 2038-22000-020-008-S
Project Type: Non-Assistance Cooperative Agreement

Start Date: May 1, 2022
End Date: Dec 30, 2023

Perform genome sequencing of current and historical Impatiens necrotic spot virus (INSV) isolates from major lettuce growing regions in California and Arizona, as well as isolates from horticultural crops, including dahlia. Understanding the genetic diversity of INSV isolates will provide greater knowledge on the biology of the virus and its potential emergence as an important pathogen of lettuce in California and Arizona.

INSV isolates have been obtained from lettuce in 2019 to 2022 in the Salinas Valley (Monterey County) and neighboring counties on the Central Coast of California (San Benito and Santa Cruz County). Isolates from the desert region in southern California (Imperial County and Coachella Valley) and Arizona (Yuma County) were also obtained from lettuce in 2021 and 2022. A second sample of INSV from 2008 was also obtained from a freezer stock at USDA in Salinas. All samples will be extracted for RNA at USDA Salinas and sent to the Cooperator (Washington State University) and sequenced to obtain genetic sequences for all the INSV isolates. Additionally, RNA will be isolated from INSV-infected dahlia and other horticultural crops by the Cooperator or USDA Salinas. All RNAs will be sequenced, processed, and analyzed by the Cooperator. At least 20 isolates will be sequenced and analyzed.