Location: Livestock Arthropod Pest Research Unit
Project Number: 3094-10400-001-011-N
Project Type: Non-Funded Cooperative Agreement
Start Date: Aug 1, 2026
End Date: Jul 31, 2028
Objective:
ARS scientist and the cooperator will advise undergraduate and graduate students in research on the genetic aspects of New World screwworm mate selection and developing novel genetic sexing strains.
Approach:
Tissues associated with mate identification and reproduction will be dissected and their RNA sequenced to identify genes of sensory receptors or production of pheromones. Once identified, CRISPR-Cas9 will be used to create genetic knockouts of target genes predicted to be involved in mating. Knockout lines will be evaluated for changes in mate success or receptivity.
The New World screwworm eradication program would greatly benefit from male-only releases. Genetically engineered male-only lines exist, but new technologies such as gene drives could further improve screwworm SIT. Cooperator will develop and evaluate novel genetic constructs with potential to improve on exisiting male-only technology. Approaches could include CRISPR-Cas9 homologous recombination, PhiC31 transposase mediated cassette exchange, or piggyBac transformation. After developing lines, they will be evaluated for expected function and suitability for use by the mass-rearing program.