Location: Crop Improvement and Protection Research
Project Number: 2038-22000-019-068-R
Project Type: Reimbursable Cooperative Agreement
Start Date: Feb 1, 2025
End Date: Nov 30, 2027
Objective:
To further develop a genomics based approach for identification of unique sequences in Fusarium oxysporum for development of diagnostic assays.
Approach:
Sequences unique to F. oxysporum f. sp. lactucae used to develop a diagnostic assay were identified using a genomics-based approach by comparison with a database of over 850 genome sequences representing 64 formae speciales of F. oxysporum. A more automated approach is under development that will simplify the process of identifying sequences to target for assay development. With the extensive genomic sequence database we have assembled (we sequenced over 450 accessions ourselves) representing a wide range of host specific pathogens the intention is to develop a systematic approach for marker development that will enable us to provide taxon specific assays for a wide range of economically important formae speciales.