Location: Cereal Crops Improvement Research
Project Number: 3060-21000-046-038-S
Project Type: Non-Assistance Cooperative Agreement
Start Date: Feb 1, 2025
End Date: Jul 31, 2027
Objective:
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1) Develop wheat lines nearly isogenic for the bacterial leaf streak resistance QTLs previously discovered on chromosomes 3B and 5A in the variety Boost and on chromosome 7D in the synthetic hexaploid line W-7984. 2) Develop recombinant inbred mapping populations segregating for individual BLS resistance QTLs and develop markers suitable for marker-assisted selection.
The objectives of this cooperative research are to: Identify candidate genes for the wheat Snn2 gene governing susceptibility to the disease septoria nodorum blotch; 2) Validate Snn2 candidate genes using mutagenesis and genetic complementation.
Approach:
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Objective 1: Wheat lines with high levels of genetic resistance to BLS are extremely rare. However, we recently identified two major QTLs for BLS resistance from the hard red spring wheat (HRSW) cultivar ‘Boost’ on chromosomes 3B and 5A and one major QTL on chromosome 7D in the synthetic hexaploid wheat (SHW) line W-7984. We will develop near-isogenic lines (NILs) for each QTL utilizing recombinant inbred lines (RIL) that exclusively possess each QTL by performing multiple backcrosses to the susceptible line ND830 to gain understanding of their individual effects against BLS in a common genetic background. This work will provide knowledge regarding the effects of each QTL under various environments, which will enhance our understanding of genetic resistance to BLS and aid wheat breeders in making informed decisions toward the development of BLS-resistant varieties.
Objective 2: The NILs developed in objective 1 will be used as parents to develop recombinant inbred populations by crossing them to ND830. These populations will each segregate for a single BLS resistance QTL and will be used for fine-mapping the QTL and the development of molecular markers suitable for marker-assisted selection, which is imperative for the deployment of the resistance QTLs into adapted wheat varieties.