Location: Sugarbeet and Potato Research
Project Number: 3060-21000-045-018-S
Project Type: Non-Assistance Cooperative Agreement
Start Date: Sep 1, 2023
End Date: Dec 30, 2024
Evaluate field soil samples for rhizomania and recover viruses that compromise rhizomania. 2)-Identify the viral variants of Beet necrotic yellow vein virus that compromise the host resistance. 3)-Determine the ability of the identified BNYVV variants in compromising host resistance provided by Rz1, Rz2, and Rz1 plus Rz2 of sugar beet varieties.
The cooperator will carry out the following: 1)-Application of high-throughput sequencing (HTS) on the root samples obtained from soil-bait sugar beet plants using different sugar beet genotypes and that includes RNA extraction, RNA-Seq library construction, and HTS using Illumina platform. 2)- Bioinformatic analyses of raw data obtained from HTS to identify the variations on the BNYVV genome (RNA1, RNA2, RNA3, and RNA4) that can compromise the host-resistance. 3)-Reverse genetics approaches to determine the ability of the identified resistance-breaking variants in compromising the host resistance provided by Rz1, Rz2, and Rz1 plus Rz2 of sugar beet varieties.