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ARS Home » Pacific West Area » Salinas, California » Crop Improvement and Protection Research » Research » Research Project #444136

Research Project: Understanding the Molecular Mechanisms of Viral Infection in Lettuce in Support of the USDA Lettuce Breeding Program

Location: Crop Improvement and Protection Research

Project Number: 2038-21530-003-015-T
Project Type: Trust Fund Cooperative Agreement

Start Date: Apr 1, 2023
End Date: Sep 30, 2024

Objective 1: To sequence the transcriptome of lettuce plants under thrip and INSV pressure in order to develop testable hypotheses for the molecular mechanisms involved in biotic stress response.

We will use inoculation methods developed by collaborators using viruliferous thrips under controlled conditions. We will sample a minimum of 3 plants of each of 4 cultivars with varying susceptibility to thrips and INSV under 3 treatments (controls that are untreated with thrips and INSV, plants that are treated with thrips only, and plants that are treated with viruliferous thrips), at 4 time periods after inoculation (12 hours post treatment, 24 hours post treatment, 72 hours post treatment, and 96 hours). Tissue will be taken from leaves and stalks, and submitted to transcriptome sequencing. Sampling is destructive, so new plants will be used for each time period sample.