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ARS Home » Pacific West Area » Hilo, Hawaii » Daniel K. Inouye U.S. Pacific Basin Agricultural Research Center » Tropical Plant Genetic Resources and Disease Research » Research » Research Project #443482

Research Project: Rapid Ohia Death - Testing and Research Support

Location: Tropical Plant Genetic Resources and Disease Research

Project Number: 2040-21000-016-039-R
Project Type: Reimbursable Cooperative Agreement

Start Date: Jan 16, 2023
End Date: Jun 30, 2024

Objective:
The objectives of the project are to provide Rapid Ohia Death (ROD) testing capacity, collect lab, greenhouse and field data, isolate ROD fungi from high priority areas, and provide information and cultures to collaborators.

Approach:
Samples will be collected by cooperators and tested for the presence of the ROD pathogens by qPCR and/or carrot baiting. Results will be added to the ROD database. Laboratory, greenhouse and/or field environments will be utilized to evaluate and characterize disease responses. Fungi will be identified using a combination of methods including light microscopy and qPCR. Fungal isolates from high priority areas will be collected, stored at the ARS culture collection in Hilo, HI, and provided to cooperators for genetic studies.