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ARS Home » Southeast Area » Raleigh, North Carolina » Plant Science Research » Research » Research Project #443218

Research Project: Phenotyping of Wheat and Barley Germplasm for Disease Resistance

Location: Plant Science Research

Project Number: 6070-22000-019-014-S
Project Type: Non-Assistance Cooperative Agreement

Start Date: Nov 1, 2022
End Date: Nov 1, 2027

The objective of this research is to phenotype advanced wheat and barley lines and cultivars from the eastern U.S. for resistance to several diseases of economic importance. The experiments are conducted to further development and release of cultivars with resistance to these diseases, which include Fusarium head blight (FHB), Septoria nodorum blotch (SNB), Cereal yellow dwarf virus (CYDV), and Pythium root rot.

Approach obj 1: For determination of FHB and SNB resistance, establish field nurseries in the fall by planting wheat and barley lines in randomized, replicated plots in multiple field locations. Appropriate practices for suppression of weeds and unwanted pathogens are followed. In the spring, prepare inoculum and inoculate with the respective pathogens. Irrigation must be provided to stimulate the development of FHB and SNB. Approach obj 2: For study of CYDV, plant field experiments in the fall, consisting of multiple wheat varieties with and without genetic resistance to CYDV. Ensure weeds are suppressed. Apply insecticides to reduce CYDV at appropriate times in November and February. Harvest at physiological maturity. Approach obj 3: For study of Pythium root rot, culture pathogen on rice kernels and inoculate wheat seedlings grown in controlled environments. Identify resistant control cultivars in order to phenotype.