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ARS Home » Midwest Area » Columbia, Missouri » Plant Genetics Research » Research » Research Project #442852

Research Project: Molecular Characterization of Soybean for Seed Quality Improvement

Location: Plant Genetics Research

Project Number: 5070-21000-045-008-S
Project Type: Non-Assistance Cooperative Agreement

Start Date: Sep 1, 2022
End Date: Aug 31, 2025

The objectives of the project: 1) Transform soybean plants with desirable traits, and 2) Characterize soybean plants containing altered genes to determine gene functions and mode of action for improving soybean seed quality.

A combination of molecular biology, system biology, genetic and genomic and physiological approaches will be employed to achieve the objectives. Specifically, DNA vectors will be constructed and transformed into soybean cell. The primary transformed soybean plantlets will be screened using PCR plus DNA sequencing for the stable transformants containing edited genes. The transcriptome sequencing of transgenic soybean seeds will be used for determining the gene functions and molecular mode of action. The seed protein and oil content, fatty acids and amino acid will be determined.