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ARS Home » Midwest Area » Columbia, Missouri » Plant Genetics Research » Research » Research Project #442568

Research Project: Refinement of Trypsin Inhibitor Measurement Procedure to Improve the Assay Consistency Leading to Industry Adoption

Location: Plant Genetics Research

Project Number: 5070-21000-044-009-R
Project Type: Reimbursable Cooperative Agreement

Start Date: Oct 1, 2022
End Date: Mar 31, 2024

Develop a simple, reliable and inexpensive procedure that can be routinely utilized by soybean processors as a standard method to measure trypsin inhibitor levels.

We recently developed an easy and rapid procedure to measure trypsin inhibitor activity in soybean meal that also reduces the assay volume from 10 to 1 mL. In this project, we will be further refining this assay and samples provided by the feed industry will be used for the validation of the new protocol. Additionally, we will examine the feasibility of measuring the trypsin inhibitor activity in 96-well microplates which will enable high-throughput screening of large number of soy meal samples.