Research Project: Molecular, Immune and Microbiome Approaches for Mitigating GI Nematode Infections of Livestock

Location: Animal Parasitic Diseases Laboratory

Publications (Clicking on the reprint icon will take you to the publication reprint.)

Transcriptomic analysis of peripheral blood mononuclear cells during Ostertagia ostertagi infection in cattle highlights a generalized host immune reaction - (Peer Reviewed Journal)
Fleming, D.S., Bakshi, M., Thompson, P.C., Beshah, E., Tuo, W. 2025. Transcriptomic analysis of peripheral blood mononuclear cells during Ostertagia ostertagi infection in cattle highlights a generalized host immune reaction. Biology. 14(8). Article e1034. https://doi.org/10.3390/biology14081034.

Disruptions in gene interaction networks abolish host susceptibility to Trichostrongylus colubriformis infections in sheep - (Peer Reviewed Journal)
Liu, F., Mcnally, J., Shao, J.Y., Ingham, A., Hunt, P., Li, R.W. 2025. Disruptions in gene interaction networks abolish host susceptibility to Trichostrongylus colubriformis infections in sheep. PLOS Neglected Tropical Diseases. 19(8). Article e0013399. https://doi.org/10.1371/journal.pntd.0013399.

Escherichia coli is implicated in the development and manifestation of host susceptibility to the roundworm Trichostrongylus colubriformis infections in sheep - (Peer Reviewed Journal)
Liu, F., Mcnally, J., Fleming, D.S., Ingham, A., Hunt, P., Li, R.W. 2025. Escherichia coli is implicated in the development and manifestation of host susceptibility to the roundworm Trichostrongylus colubriformis infections in sheep. Veterinary Research. 56. Article e133. https://doi.org/10.1186/s13567-025-01565-1.

Transcriptional profiling of abomasal mucosa from young calves experimentally infected with Ostertagia ostertagi - (Peer Reviewed Journal)
Boschiero, C., Beshah, E., Bakshi, M., Miramontes, E.N., Hebert, D.A., Thompson, P.C., Li, C., Zhu, X., Zarlenga, D., Liu, G., Tuo, W. 2025. Transcriptional profiling of abomasal mucosa from young calves experimentally infected with Ostertagia ostertagi. International Journal of Molecular Sciences. 26(5). Article e2264. https://doi.org/10.3390/ijms26052264.

Wise Roles and Future Visionary Endeavors of Current Emperor: Advancing Dynamic Methods for Longitudinal Microbiome Meta-Omics Data in Personalized and Precision Medicine - (Literature Review)
Oh, V.S., Li, R.W. 2024. Wise Roles and Future Visionary Endeavors of Current Emperor: Advancing Dynamic Methods for Longitudinal Microbiome Meta-Omics Data in Personalized and Precision Medicine. Briefings in Bioinformatics. 11(47). Article e2400458. https://doi.org/10.1002/advs.202400458.

Transcriptional responses consistent with perturbation in dermo-epidermal homeostasis in septic sole ulceration - (Peer Reviewed Journal)
Reeder, T., Zarlenga, D., Ziegler, A., Dyer, R. 2024. Transcriptional responses consistent with perturbation in dermo-epidermal homeostasis in septic sole ulceration. Journal of Dairy Science. 107(10):8432-8451. https://doi.org/10.3168/jds.2023-24578.

Molecular evidence of sterile tissue damage during pathogenesis of the pododermatitis aseptica hemorrhagica circumscripta is associated with disturbed epidermal-dermal homeostasis - (Peer Reviewed Journal)
Reeder, T., Zarlenga, D., Dyer, R. 2024. Molecular evidence of sterile tissue damage during pathogenesis of the pododermatitis aseptica hemorrhagica circumscripta is associated with disturbed epidermal-dermal homeostasis. Journal of Dairy Science. 107(10):8413-8431. https://doi.org/10.3168/jds.2023-24577.

Differentiation and regulation of bovine Th2 cells in vitro - (Peer Reviewed Journal)
Kandel, A., Li, L., Wang, Y., Tuo, W., Xiao, Z. 2024. Differentiation and regulation of bovine Th2 cells in vitro. Cells. 13(9). Article e13090738. https://doi.org/10.3390/cells13090738.

Phospholipid-rich krill oil promotes intestinal health by strengthening beneficial gut microbial interactions in an infectious colitis model - (Peer Reviewed Journal)
Liu, F., Smith, A.D., Wang, T.T., Pham, Q., Hou, P., Cheung, L., Yang, H., Li, R.W. 2024. Phospholipid-rich krill oil promotes intestinal health by strengthening beneficial gut microbial interactions in an infectious colitis model. Food & Function. 15(5):2604-2615. https://doi.org/10.1039/D3FO04980A.

The identification of small molecule inhibitors with anthelmintic activities that target conserved proteins among ruminant gastrointestinal nematodes - (Peer Reviewed Journal)
Jung, H., Zarlenga, D., Martin, J.C., Geldhof, P., Hallsworth-Pepin, K., Mitreva, M. 2024. The identification of small molecule inhibitors with anthelmintic activities that target conserved proteins among ruminant gastrointestinal nematodes. mBio. 15(3). Article e00095. https://doi.org/10.1128/mbio.00095-24.

Discovery of new broad-spectrum anti-infectives for eukaryotic pathogens using bioorganometallic chemistry - (Peer Reviewed Journal)
Lin, Y., Jung, H., Bulman, C.A., Ng, J., Vinck, R., O'Beirne, C., Moser, M.S., Tricoche, N., Peguero, R., Li, R.W., Urban, J., Le Pape, P., Pagniez, F., Moretto, M., Weil, T., Lustigman, S., Mitreva, M., Sakanari, J.A., Gassera, G. 2023. Discovery of new broad-spectrum anti-infectives for eukaryotic pathogens using bioorganometallic chemistry. Journal of Medicinal Chemistry. 66(23):15867-15882. https://doi.org/10.1021/acs.jmedchem.3c01333.

An FcRn-targeted mucosal vaccine against SARS-CoV-2 infection and transmission - (Peer Reviewed Journal)
Li, W., Wang, T., Rajendrakumar, A.M., Acharya, G., Miao, Z., Varghese, B.P., Yu, H., Dhakal, B., Leroith, T., Karunakaran, A., Tuo, W., Zhu, X. 2023. An FcRn-targeted mucosal vaccine against SARS-CoV-2 infection and transmission. Nature Communications. 14(1). Article e7114. https://doi.org/10.1038/s41467-023-42796-0.

Differential correlation of transcriptome data reveals gene pairs and pathways involved in treatment of Citrobacter rodentium infection with bioactive punicalagin - (Peer Reviewed Journal)
Fleming, D.S., Liu, F., Li, R.W. 2023. Differential correlation of transcriptome data reveals gene pairs and pathways involved in treatment of Citrobacter rodentium infection with bioactive punicalagin. Molecules. 28(21). Article e28217369. https://doi.org/10.3390/molecules28217369.

Rumen microbiome dynamics and their implications in health and environment - (Peer Reviewed Journal)
Li, R.W., Moon, C.D., Morgavi, D.P. 2023. Rumen microbiome dynamics and their implications in health and environment. Frontiers in Microbiology. 14. Article e1223885. https://doi.org/10.3389/fmicb.2023.1223885.

After bone marrow transplantation, cell-intrinsic T Helper-2 (Th2) pathway promotes recipient T lymphocyte survival and regulates the graft-versus-host disease - (Peer Reviewed Journal)
Truscott, J., Guan, X., Fury, H., Atagozli, T., Metwali, A., Liu, W., Li, Y., Urban Jr, J.F., Li, R.W., Brombacher, F., Elliott, D., Blazer, B., Ince, M. 2023. After bone marrow transplantation, cell-intrinsic T Helper-2 (Th2) pathway promotes recipient T lymphocyte survival and regulates the graft-versus-host disease. Journal of Immunology. https://doi.org/10.4049/immunohorizons.2300021.

Multi-omics analysis detected multiple pathways by which pomegranate punicalagin exerts its biological effects in modulating host–microbiota interactions in murine colitis models - (Peer Reviewed Journal)
Liu, F., Smith, A.D., Wang, T.T., Pham, Q., Yang, H., Li, R.W. 2023. Multi-omics analysis detected multiple pathways by which pomegranate punicalagin exerts its biological effects in modulating host–microbiota interactions in murine colitis models. Food & Function. 14:3824. https://doi.org/10.1039/D3FO00286A.

Ellagitannin punicalagin disrupts the pathways related to bacterial growth and affects multiple pattern recognition receptor signaling by acting as a selective histone deacetylase inhibitor - (Peer Reviewed Journal)
Liu, F., Smith, A.D., Wang, T.T., Pham, Q., Yang, H., Li, R.W. 2023. Ellagitannin punicalagin disrupts the pathways related to bacterial growth and affects multiple pattern recognition receptor signaling by acting as a selective histone deacetylase inhibitor. Journal of Agricultural and Food Chemistry. 71(12):5016-5026. https://doi.org/10.1021/acs.jafc.2c08738.

Biological pathways via which the anthocyanin malvidin alleviated the murine colitis induced by Citrobacter rodentium - (Peer Reviewed Journal)
Liu, F., Smith, A.D., Wang, T.T., Pham, Q., Cheung, L., Yang, H., Li, R.W. 2022. Biological pathways via which the anthocyanin malvidin alleviated the murine colitis induced by Citrobacter rodentium. Food & Function. 14(2):1048-1061. https://doi.org/10.1039/D2FO02873E.

Recent advances in developing butyrogenic functional foods to promote gut health - (Review Article)
Zhang, M., Li, R.W., Yang, H., Tan, Z., Liu, F. 2022. Recent advances in developing butyrogenic functional foods to promote gut health. Critical Reviews in Food Science and Nutrition. 1-22. https://doi.org/10.1080/10408398.2022.2142194.

Horizontal gene transfer provides insights into the deep evolutionary history and biology of Trichinella - (Literature Review)
Zarlenga, D., Thompson, P.C., Mitreva, M., Rosa, B., Hoberg, E. 2022. Horizontal gene transfer provides insights into the deep evolutionary history and biology of Trichinella. Food and Waterborne Parasitology. https://doi.org/10.1016/j.fawpar.2022.e00155.