Location: Plant, Soil and Nutrition Research
Project Number: 8062-21000-047-032-R
Project Type: Reimbursable Cooperative Agreement
Start Date: Aug 1, 2021
End Date: Apr 14, 2023
The project goal is to identify a cohort of candidate genes/proteins that could be targeted for improving pollen heat tolerance traits. The four objectives are: 1). Profiling the heat-induced transcriptomes and proteomes of the following tissues: a) pollen mother cells (PMC) at meiosis, tetrads, and microspore stages; b) tapetal cells from the same tissue section; and c) viable and non-viable pollen separately; 2). Identifying pollen heat stress genes/proteins through systemic analysis of the transcriptome/proteome datasets, and 3). Developing methods for validation of gene (transcript and protein) expression and biomarkers of heat tolerance; 4). Using these results to prepare a research proposal, tentatively entitled, “Developing heat tolerant tomatoes using CRISPR targeting on pollen heat stress genes”, and submit to the NIFA program.
Specific activities performed in the ARS labs will include 1) a comparative proteomic analysis of treated and control plant material using a 10-plex TMT strategy on pollen mother cells (PMC) at meiosis, tetrads, and microspore stages, tapetal cells from the same tissue section and viable and non-viable pollen; 2) Students from Tennessee State University will be trained in proteomics at the RW Holley Center for Agriculture and Health through their participation in these research activities.