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ARS Home » Midwest Area » Columbia, Missouri » Plant Genetics Research » Research » Research Project #439683

Research Project: Characterize rootworm larvae for bt tolerance and artificial diets for improved understanding of rootworm biology and management

Location: Plant Genetics Research

Project Number: 5070-21000-041-24-S
Project Type: Non-Assistance Cooperative Agreement

Start Date: Dec 1, 2020
End Date: Nov 30, 2021

1) Characterize heritability and other traits of rootworm larvae with Bt tolerance.

Newly emerged virgin male and female western corn rootworm adults from both Cry34/35Ab1-selected colonies and their control colony will be collected and isolated from each other. Reciprocal cross colonies will be created by combining at least 100 Cry34/35Ab1-selected males with 100 control females and vice versa for each from both Cry34/35Ab1-selected colonies. Eggs from these colonies will be collected and tested along with those of the parental Cry34/35Ab1-selected and control colonies in diet-toxicity and plant assays similar to those that we have published previously.