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ARS Home » Plains Area » Fargo, North Dakota » Edward T. Schafer Agricultural Research Center » Sunflower and Plant Biology Research » Research » Research Project #439457

Research Project: Enhancing Rust Resistance in Confection Sunflower Production Through Next-generation Technologies - Year 2

Location: Sunflower and Plant Biology Research

Project Number: 3060-21000-043-32-T
Project Type: Trust Fund Cooperative Agreement

Start Date: Oct 1, 2020
End Date: Sep 30, 2022

Objective:
Objective 1: Stack effective rust R genes to generate multi-disease resistant lines. Objective 2: Construct high density SNP genetic maps of three rust R genes using whole genome re-sequencing and fine mapping approaches. Objective 3: Develop diagnostic molecular marker for the rust R genes, R12, R13a, and R15. Objective 4: Identify candidate gene(s) associated with rust resistance in the sunflower genome.

Approach:
We will apply cutting edge genetic and genomic approaches to characterize the genetic basis for rust resistance in sunflower. With improved next generation sequencing (NGS) and the decreased cost of sequencing, it is now feasible to discover millions of SNPs for any plant and connect these markers to desirable phenotypic traits. We will use whole genome re-sequencing combining the fine mapping approach to generate high density SNP genetic maps for characterization of the rust R genes, R12, R13a, and R15, to dissect the R gene cluster, and to identify candidate genes for rust resistance. The diagnostic SNP markers linked to the rust R genes will be evaluated across a diversity panel of common breeding parents and optimized prior to public release. Project deliverables include new tools (i.e. SNP markers) that will enable sunflower breeding programs across the US to more easily develop superior cultivars that are resistant to rust. Ultimately, these efforts will result in the development of improved rust resistant hybrids, providing a more economically sustainable solution for confection sunflower growers throughout the US and the world.