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ARS Home » Midwest Area » Ames, Iowa » National Animal Disease Center » Food Safety and Enteric Pathogens Research » Research » Research Project #439239

Research Project: Development of New Swine Reagents to Broaden Our Understanding of Immune Cell Identity and Function

Location: Food Safety and Enteric Pathogens Research

Project Number: 5030-32000-225-007-R
Project Type: Reimbursable Cooperative Agreement

Start Date: Jul 15, 2019
End Date: Jul 14, 2022

Objective:
1. Develop and characterize panels of monoclonal antibodies (mAb) specific for swine immune proteins, e.g., cytokines. 2. Generate swine leukocyte antigenclass I tetramers for several common US pig breeds 3. Express specific transcription factors regulating critical immune interactions and develop/identify mAbs to them 4. Generate/identify mAbs specific for porcine cluster differentiation (CD) antigens 5. Identify commercial partners for downstream use of developed reagents.

Approach:
ARS will focus on Objectives 3 and 4. Objective 3 and 4 targets will include characterizing mAbs produced by commercial partners. This may also include analyses of mAbs produced during a previous collaboration. Western blots and flow cytometry will be used to confirm specificity of mAbs, define epitopes recognized and cross species reactivities. Intracellular staining will be tested on pig cell subsets as appropriate.