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ARS Home » Northeast Area » Ithaca, New York » Robert W. Holley Center for Agriculture & Health » Emerging Pests and Pathogens Research » Research » Research Project #438922

Research Project: Mechanisms of Compatible and Incompatible Interactions Between Potato and Potato Cyst Nematodes

Location: Emerging Pests and Pathogens Research

Project Number: 8062-22000-022-028-A
Project Type: Cooperative Agreement

Start Date: Aug 1, 2020
End Date: Jul 31, 2025

Objective:
1. Determine the resistance of potato breeding clones, varieties and wild potato species to G. rostochiensis pathotypes. 2. Evaluate responses of wild potato accessions to nematode infection, generate mapping populations and downstream mapping and identifying novel resistance genes from wild potato clones. 3. Analyze and characterize nematode effector proteins and their host targets contributing to disease or plant resistance.

Approach:
1. Greenhouse bioassays will be conducted to determine the resistance of potato breeding clones, varieties and wild species to G. rostochiensis Ro1 and Ro2. 2. Histological analysis will be used to evaluate responses of wild potato clones to nematode infection, genetic crosses will be made to generate mapping populations, which will be used for identifying novel resistance genes through RenSeq and long-read sequencing. 3. Molecular biology and functional genomics approaches will be used to elucidate the function of effector proteins and their interacting host proteins in plant parasitism or host resistance.