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ARS Home » Southeast Area » Charleston, South Carolina » Vegetable Research » Research » Research Project #438177

Research Project: Development of Genetic Resources for Host-plant Resistance to Leaf Blight Pseudomonads in Turnip Greens and Spinach

Location: Vegetable Research

Project Number: 6080-22000-028-32-R
Project Type: Reimbursable Cooperative Agreement

Start Date: Jun 1, 2020
End Date: May 31, 2023

Objective:
To reduce the impact of bacterial leaf blight (caused by the phytobacteria Pseudomonas spp.) on Brassica leafy greens and spinach by use of plant host resistance.

Approach:
The use of biological assays and genomics will be employed to identify resistant Brassica and spinach germplasm, and to further identify quantitative trait loci (QTL) associated with the resistance, and finally designing molecular-based markers for use by industry breeders for introgression of this resistance into their elite lines by marker assisted breeding. The ARS PI will use biological assays to first identify germplasm with resistance to the leaf blight bacterial pathogen both in greenhouse and field conditions. Selections from these screens will be taken to advanced generation by grant collaborators. Each new generation will be again tested for resistance by ARS PI. ARS PI will assist in development and validation of molecular markers.