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ARS Home » Pacific West Area » Hilo, Hawaii » Daniel K. Inouye U.S. Pacific Basin Agricultural Research Center » Tropical Pest Genetics and Molecular Biology Research Unit » Research » Research Project #436671

Research Project: 6R.0392.00 Expanding Non-Transgenic Genetic Sexing Systems to New Pest Tephritid Species

Location: Tropical Pest Genetics and Molecular Biology Research Unit

Project Number: 2040-22430-028-017-I
Project Type: Interagency Reimbursable Agreement

Start Date: Jul 1, 2019
End Date: Oct 30, 2022

Develop non-transgenic genetic sexing strains in pest tephritid species to expand the application of SIT in this important pest species group.

Characterization of mutations conferring white pupae and temperature sensitive lethal in medfly genetic sexing strains, as well as characterization of the exact translocation breakpoint between the Y chromosome and the 5th autosome in the males of the Veinna-8 strain that confers these traits to be female specific. From that develop CRISPR-based methods to recreate the translocation in wild type lines, and demonstrate a targeted gene editing approach to re-creating temperature sensitive lethal in Anastrepha or Bactrocera species.