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ARS Home » Pacific West Area » Hilo, Hawaii » Daniel K. Inouye U.S. Pacific Basin Agricultural Research Center » Tropical Crop and Commodity Protection Research » Research » Research Project #436671

Research Project: 6R.0392.00 Expanding Non-Transgenic Genetic Sexing Systems to New Pest Tephritid Species

Location: Tropical Crop and Commodity Protection Research

Project Number: 2040-22430-026-45-I
Project Type: Interagency Reimbursable Agreement

Start Date: Jul 1, 2019
End Date: Oct 30, 2022

Objective:
Develop non-transgenic genetic sexing strains in pest tephritid species to expand the application of SIT in this important pest species group.

Approach:
Characterization of mutations conferring white pupae and temperature sensitive lethal in medfly genetic sexing strains, as well as characterization of the exact translocation breakpoint between the Y chromosome and the 5th autosome in the males of the Veinna-8 strain that confers these traits to be female specific. From that develop CRISPR-based methods to recreate the translocation in wild type lines, and demonstrate a targeted gene editing approach to re-creating temperature sensitive lethal in Anastrepha or Bactrocera species.